Techniques

For scientific training

List of techniques used for scientific training by students of the the research teams: Genetics of the brain, Synaptic interactome and Functional genomics to address their aims in all the research teams.

Neuronal culture:

  • Organotypic culture (slices)
  • Primary culture (neurons, glia)
  • Trans/Infection/ siRNA technology

Molecular biology:

  • Generation viral mediated expression constructs
  • Stereotaxic injections/viral gene transfer
  • Generation of epitope tagged proteins
  • Purification of tagged fusion proteins
  • Site-directed mutagenesis
  • Large-scale automated sequencing
  • Generation of knock-out/transgenic mice
  • mRNA/RNA translational assays
  • mRNA/RNA stability assays

Gene / Protein profiling:

  • Real-time quantitative PCR
  • Construction expression libraries
  • Massive Parralel Sequencing.
  • Data analysis
  • Mass spectrometry (LC-LC MS-MS)

Protein chemistry:

  • Electrophoresis: 1D-, 2D-PAGE
  • Immunoprecipitation/Western blot
  • Postsynaptic membrane fractionation
  • Surface Plasmon Resonance
  • Protein phosphorylation assays

Neural imaging:

  • Evanescent Wave Microscopy
  • Immunofluorescence
  • Digital Electron Microscopy
  • Morphometry
  • 2-photon microscopy
  • Spot confocal microscopy
  • High temporal resolution confocal microscopy

Brain genetics:

  • STR and SNP genotype analysis
  • Positional cloning, LD analysis
  • Quantitative genome analysis/MLPA
  • Clinical diagnosis, neuropathology, linkage, association analysis, statistics

Electro-physiology:

  • Current and voltage patch clamping
  • Functional imaging (photodiode array)
  • Multi-array recordings
  • ECG monitoring

Computer science:

  • Modelling of neural networks and protein complexes
  • Quantal analysis/mathematical modelling
  • Development of new (non-linear) algorithms for behavioural phenotyping

Behaviour

  • High/Low throughput phenotyping
  • Autonomic readouts