Yokohama City, 16 – 18 September 2013
Course: Identifying regulatory elements in the genome
This course focuses on the use of genomic tools to identify regulatory elements in the genome, such as genes, transcription factors and non-coding RNAs. We discuss the use of RNA sequencing and other kinds of tagging-based RNA methods, such as CAGE (Cap Analysis Gene Expression), for capturing regulatory elements. In particular we will focus on the basic experimental principles and experimental design strategies for CAGE and discuss the use of high throughput sequencing in these experiments.
The interpretation of high throughput sequencing data is a challenging task that requires the use of bioinformatic tools. We will outline commonly used bioinformatic tools, methodologies and pipelines that allow us to study sequencing data. Lastly, we will provide examples of the use of such technologies in answering biological questions. >Course Schedule
Yokohama City, 19 September 2013
Neuroscience BrainTrain Conference
See >Program BrainTrain Conference, Yokohama, Japan
For more information see: www.clst.riken.jp/event/2013/BrainTrain.html
Yokahama City, 20 September 2013
Paris, April 2013
Electrophysiolical recording and imaging
The first two days will be lectures. Day 3: 6 practical experiments with electrophysiological recording and imaging, and demonstrations in local labs.
The practical experiments:
- Extracellular spike recording and analysis
- Patch clamp recording and stimulation in slices.
- CCD imaging of nerve tract excitation and postsynaptic recording
- Voltage sensitive indicators of membrane potential
- Whole cell recording and CCD imaging of Ca concentration
- Dendritic patch clamp recording
- Perforated patch recording
Leuven, April 2013
mRNP transport in neurons
mRNP transport in neurons and synaptic plasticity (15 persons) Day 1: Morning: 3 lectures on RNA/protein transport and synaptic plasticity. Afternoon: Preparation of synaptosomes. Day 2 and 3 Analysis of synaptic RNAs or proteins (to be decided).