London, May 2-4th 2012
The aim of this course is to provide an introduction to 3D biophysically detailed neuronal modelling and recent advances in 2-photon imaging methods for studying rapid neuronal signalling. The course will give an overview of the field of detailed compartmental modelling and how it can complement experimental approaches including 2-photon imaging. It will consist of some introductory lectures on modelling and imaging, provide hands on experience of using neuroConstruct, introduce the existing cortical, cerebellar and hippocampal models which are already available in this format, and outline the process of creating new models from experimental data. The course will also cover the simulator independent model description language NeuroML and the growing number of tools available for handling models in this format.
Participants: 40 (including 18 researchers)
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Barcelona, 12-13th July
Joint meeting BrainTrain, CerebNet and Enc Network, satellite Meeting of the Fens Meeting 2012
Hotel Front Marítim Barcelona, Pº García Faria, 69, 08019 BARCELONA, SPAIN, Tel. +34 933 034 440, www.gbbhotels.com
Programme day 1
- Introduction to ENC Network / BrainTrain / Cerebnet / SyMBaD
- Blitz presentations projects participating networks
- Transferable skills lectures
Programme day 2
- Selected oral reports participating networks
- Poster market and award (students are informed who will contribute to the poster market)
- Transferable skills lectures
- Keynote lecture Prof. dr. Guus Smit
Trieste, 1-5 October 2012
Molecular basis of Neurodegeneration
Content: Neurogenomics : This course will provide an overview of all the techniques involved in the study of gene expression in the brain including cell labelling, cell purification, RNA amplification, microarray and deep-sequencing.
Content: Molecular basis of Neurodegeneration: This conference will discuss the recent advances in neurodegenerative diseases with special emphasis on the mechanisms of cell type-specific susceptibility and gene expression profiles experiments in post mortem brains as well as in mouse models of the disease.
Participants: 65 (including 25 researchers)
Trieste, 5 October 2012
Magdeburg, 12-18 December 2012
Finding molecular keys to brain dysfunction
Content: Bottom-up or Top-down: how to find molecular keys to brain dysfunction
Day 1: lectures concerning “Molecules, Synapses, Networks – Basic Principles of Neural Organization”.
Following days: Bottom-up approaches like mouse mutants for synaptic proteins and Top-down techniques like proteomics of the dysfunctional brain or Imaging genetics: how genes influence brain structure or behaviour in animals or humans.
Participants: 50 (including 18 researchers)
Heidelberg , 19 – 20 December 2012
Supraresolution imaging of cells and tissues
Content: Topic is targeted perturbations of synaptic function using viral gene transfer and mouse genetics. This includes:
- methods of perturbations (KO, RNAi, toxins, dominant-negatives, silencers, and actuators);
- viral gene transfer systems;
- stereotaxic approaches;
- techniques of functional exploration of perturbations;
- techniques for detection of structural alterations;
- quantitative image processing;
- determination of gene expression profiles and compensatory changes caused by perturbations;
- modeling and simulation.
Practical ‘didactic’ lectures will alternate with research talks presenting latest results using these technologies.
Practical demonstrations are organized in the laboratory if the total number of participants permits.
Arrival on 18 December from Magdeburg, departure 20 December
Mo: 9:00 to 17:00 Lectures; 18:30 Dinner
Tue: 9:00 to 12:00 Lectures; 13:00 to 17:00 Practical demonstrations
Workload 15h = 0.6 ECTS
Heidelberg, 21-23 December 2012
Targeted molecular perturbation course
“Perturbation of synaptic function” is designed as a practical course for a maximum of 6 students (workload of 40h) with a focus on stereotaxic methods (anesthesia, surgery, cartesian coordinate system & atlases, validation of target area) and viral gene transfer (AAV production and purification).
- Methods of perturbations (KO, RNAi, toxins, dominant-negatives, silencers, and actuators)
- Viral gene transfer systems
- Stereotaxic approaches
- Structural and functional exploration of perturbations
- Practical part, learn how to
- Anesthesize rodents
- Perform stereotaxic surgery
- Stereotaxically deliver reagents into the brain
- Evaluate stereotaxic injection sites
- Design stereotaxic delivery experiment
Wed: 9:00 to 12:00 Lectures; 14:00 to 18:00 practicals
Thu: 9:00 to 18:00 practicals
Fri: 9:00 to 12:00 practicals; 14:00 to 15:00 discussion
Dates: 21.12 to 23.12. (after winter school, departure 23.12. afternoon)
Workload 22h = 0.8 ECTS
Budget: 500 EUR (mice and reagents)