Who hasn’t experienced the following?
You set up a new line of research and need to use a new method. You buy all the equipment needed, as written in the research article where you learned about the technique and try to adapt the method to your line of research. And it turns out that nothing works….
It always seems there is some essential bit of the technique missing or a minor detail that has been overlooked in the protocol. But what is it that you’ve missed? Given that you used a well-written paper, it is usually the little things that cannot be written down but can only be shown to you practically that are missing, thus preventing the technique from working in your own lab.
JoVE, the Journal of Visualized Experiments, gives the opportunity to share a method with the science community by actually showing how to prepare and perform an experiment. We used the opportunity to create a video-methods paper of the well-established calcium imaging technique in immature brain slices.
At the same time, it allowed us to share a couple of experience-based improvements to the method that we developed in the lab during the last two years.
We hope this movie gives those new to imaging techniques an insight into the detail of how these experiments are carried out. Additionally, we are sharing a couple of the in-house developed improvements to the attention of fellow imaging researchers.